The research was conducted to determine the number and species composition of microorganisms isolated from the soils collected in the vicinity of seven largest transport nodes and roundabouts in Krakow. Moreover, the investigations aimed at verifying the differences in the occurrence and biodiversity of the researched microorganism population between four calendar seasons of the year. The soil samples were collected 4 times during the period from November 2013 to August 2014 and then analysed using serial dilutions method. A great microorganism biodiversity was found in the analysed samples. Microorganisms were the most numerous in the autumn-winter period. The most numerous isolated group were vegetative bacteria and ammonifiers. Filamentous fungi were less numerous, which may have been caused by the neutral or slightly alkaline soil pH. Presence of dormant bacteria forms may result from unfavourable environmental conditions caused by a toxic effect of the substances from road transport. Less numerously isolated were actinomycetes and Azotobacter bacteria, regarded as bioindicators of soil fertility. It was found, that the differences in the numbers of the analysed microorganisms over the year are statistically significant for the vegetative and ammonifying bacteria, phytopathogenic fungi and actinomycetes. No significant differences in the microorganism number were stated ...
One of the most frequently applied methods of mixed municipal solid waste biological treatment in mechanical-biological treatment installations (MBT) is their disposal in aerobic biostabilization process. The process comprises an intensive phase and maturation phase. The intensive phase relies on waste heating in result of organic matter breakdown conducted by microorganisms settling the wastes. Microorganisms living in wastes have optimal conditions for development, i.e. a considerable space volume, optimal material fragmentation and organic matter availability. The aim of the aerobic biostabilization process is stabilizing and hygienization of wastes, so that they become a valuable raw material for other recovery processes (e.g. RDF production) or their disposal is safe for the environment and do not pose any epidemiological hazard for people employed in waste treatment plants.Analyses presented in the paper aimed to determine the number and species composition of vegetative and endospore bacteria, mold fungi, actinomycetes and pathogenic microorganisms, i.e. Staphylococcus spp., E. coli, Salmonella spp., Shigella spp., E. faecalis, C. perfringens, settling municipal wastes prior to and after aerobic stabilization process. The aerobic stabilization process (intensive phase) was conducted in a laboratory BKB 100 bioreactor using the wastes, characterized by low share of biodegradable wastes (<40 %), obtained from MBT ...
Celem pracy była ocena bakteriobójczych właściwości nanocząstek srebra w stosunku do bakterii Escherichia coli. Ponadto, wykonano analizę wrażliwości bakterii E. coli na powszechnie stosowane antybiotyki. W przeprowadzonych badaniach wykorzystano 51 szczepów izolowanych z odpadów komunalnych. Zastosowano wzrastające stężenia nanosrebra: 5, 10, 15, 30, 60 oraz 125 ppm. Kontrolą pozytywną był antybiotyk - ampicylina, negatywną - woda destylowana. Analizę lekooporności wykonano przy pomocy metody dyfuzyjno-krążkowej. Wraz ze wzrostem stężenia nanocząstek srebra, wzrastały ich właściwości antybakteryjne. Najmniejszym stężeniem hamującym wzrost niektórych izolatów było 5 ppm, natomiast najskuteczniejsze było stężenie 125 ppm. Analiza lekooporności wykazała, że antybiotykiem hamującym wzrost największej liczby szczepów E. coli była amikacyna. Ponadto, wykryto szczepy wielolekooporne stanowiące 22%. Nanosrebro posiada duży potencjał bakteriobójczy i może okazać się pomocne w rozwiązaniu problemu lekooporności. Jednak, zasadne jest prowadzenie dalszych badań oceniających wpływ toksyczności nanocząstek na środowisko i organizmy wyższe. ...
Apart from the basic input, the right course of fermentation process is determined by the appropriate microorganism populations and the parameters such as: pH, particle size, ionic strength (salinity) and concentration of nutrients and toxic compounds. Three microorganism groups are involved in the process of anaerobic transformation of organic substances in fermentation gas: acid forming bacteria, acetate bacteria and methanogenic bacteria. The first two phases are dominated by the bacteria which are both obligate, facultative and absolute anaerobes (Clostridium spp., Bifidobacterium spp., Streptococcus spp., Enterobacter spp., Bacillus spp., Pseudomonas spp., Aerobacter spp., Alcaligenes spp, Escherichia spp, Lacto¬bacillus spp, Micrococcus spp. czy Flavobacterium spp.) [Nimmrichter, Kuebler, 1999]. Their number during the mesophile fermentation is estimated for between 108-109 per 1ml [Hartman, 1999]. The rate of bacteria growth at both phases fluctuates from 5 hrs, in the presence of carbohydrates to 72 hrs during fat decomposition [Heidrich, Nieścier, 1999]. Presented research aimed at determining the quantity and quality of biogas depending on the microbiological environment in fractions originating from agriculture and agro-food industry. To achieve the objective of research, was evaluated the number and biodiversity of microorganisms that inhabit selected agricultural raw materials used in the production of biogas. A very important ...
Illegal waste deposition in places unadapted for this purpose poses a hazard to the natural environment. Each year over 10 000 illegal dumping sites are liquidated in Poland. The areas where illegal landfills occur are most frequently situated in the vicinity of waste management plants, wastelands, roadsides, byways or unfenced private plots (particularly on the city outskirts), forests and water courses. Despite of the changes introduced in recent years in the system of municipal solid waste management in Poland, which in the first place aimed to prevent formation of illegal dumps, such places still arise causing pollutant emission into the atmosphere. The aim of the paper was conducting an analysis of the existing illegal dumping sites in Krosno commune (Podkarpackie voivodship). The investigations were conducted along the selected stretches of the Wisłok and Lubatówka rivers. The analyses were carried out in three measurement series during the period from March to October 2014. As results from the analyses, new illegal dumping sites arise all the time in the water courses in Krosno city and a majority of them can be easily identified in this area. Glass and plastics have the biggest share in the morphological composition of the waste deposited on ...
