The adverse effect of roads on the environment is observed both during their construction and use. Increasing areas of farmland and forestland are subjected to transformations disadvantageous for animals. It is connected with the loss of natural habitats, changes in water relations and fragmentation of forest complexes. The possibility of free migration of representatives of a given species is the basis for the sustainable functioning of a population. In order to provide ecological communication of an area divided by a road or railway more and more often crossings for animals are being build over or under this road or line. The aim of the investigations was to determine the acceptance level by migrating game of two overpasses built over communication routes: a road and a railway line. Investigations were conducted on a crossing for animals over trunk road no. 5 in the Wielkopolski National Park and on the two newly constructed crossings over a modernized railway line E 20 at the Rzepin – Kunowice section. The effectiveness of crossings over the railway line was assessed by tracking. The crossing over the road was monitored using a specially designed filming unit. Analyses of the operating crossings for animals over railway line ...