The aim of the study was to assess the drug resistance profile of E. coli bacteria isolated from municipal waste. It was visible whether among the collected isolates there are strains resistant to ESBL mechanisms (extended spectrum β-lactamase) and whether they are resistant to β-lactam antibiotics. Analysis of the use of disk diffusion methods, using 7 final results for β-lactams (including 2 penicillins, 5 cephalosporins). The obtained comparative results with the KORLD (National Reference Center for Antimicrobial Susceptibility) and EUCAST (European Committee for Antimicrobial Susceptibility Testing) standards. The analysis distinguished that among 70% of isolates there is no resistance marking to unconfirmed from the tested antibiotics. On the contrary, 30% of strains were resistant to more than 1 antibiotic. Intermediate susceptibility to cefazolin is characteristic of 80% of E. coli strains. The largest number, as many as 20% of strains, were resistant to this antibiotic. In contrast to 100% susceptibility compared to amoxicillin with clavulanic acid, which should be used for penicillin. The ESBL mechanism is not a habitat. It has been found that municipal waste is a place of residence of E. coli bacteria resistant to antibiotics. It is very important to continue research on this source of drug ...
Air analysis was carried out in selected vegetable storage rooms to assess the level of contamination by indicator microorganisms - general bacteria, fungi, staphylococci and actinomycetes. The air quality inside the storage facilities was compared with the external environment (control points near the storage facilities). The analysis also included the physical factor of air pollution, the PM2.5 and PM10 dust fractions. The tests were carried out over eight months: before the start of vegetable storage in the storage facilities, at the beginning, during and at the end of this period. It was planned to verify the existence of a relationship between the concentration of microorganisms and the other variables being determined. Due to the lack of applicable standards, the determined numbers of microorganisms were referred to the reference values from legal acts withdrawn in 2015. The results regarding dust concentration were compared with the applicable Regulation of the Minister of the Environment of 24 August 2012 and the air quality index of the Chief Inspectorate for Environmental Protection. As this study has shown, the environment of vegetable storage facilities is inhabited by potentially dangerous microorganisms, mainly mould fungi, and high dust levels, which raises doubts in the context of the ...
