Construction of biogas plants processing agricultural waste enables to reduce waste, limit emission of gases and odors in rural areas as well as gives the possibility to ensure self-sufficiency of energy. The amount of generated electricity and heat depends on the quantity and quality of biogas. The aim of this work was to show how the substrate affects the amount and composition of the biogas in agricultural biogas plants. The most commonly applied types of substrates in agricultural biogas plants in 2011-2012 and in the first half of 2013 were indicated due to the Agricultural Market Agency data. The research also showed the amount of agricultural biogas produced as well as the amount of electricity and heat generated from agricultural biogas during the time period mentioned above.